Principal Investigator

饶毅
行为和社会认知、细胞和分子机制、化学连接组学

联系电话：
通信地址： 北京市海淀区颐和园路5号
电子邮件：yrao@pku.edu.cn

 

研究兴趣：

  人体内最重要的信号分子是神经系统的神经递质。它们负责神经细胞之间、神经细胞与靶细胞之间的信息传输。没有神经递质，人就不可能思考。科学界普遍认为神经递质几十年前就基本都找到了。本实验室认为很可能还有脑内的其它神经递质，因为以前找到的神经递质依赖神经系统之外的检测方式。本实验室用生物化学方法分离含神经递质的亚细胞结构。从中分离、分析化学分子。之后进一步确定是否是新的神经递质分子。
  睡眠的重要性无需赘述，但睡眠的机理了解有限。本实验室通过遗传筛选和生化分离等途径寻找可能影响睡眠的分子。通过改变分子活性追踪它们调控睡眠的功能。
  GPCR是最常见的受体分子，被内源配体分子激活。它们同时也是最常见的药物靶点。本实验室寻找受体新的配体，并研究其生理和病理功能。
  蛋白质在蛋白激酶作用下磷酸化，在磷酸酶作用下去磷酸化。蛋白质磷酸化调控体内很多功能。本实验室通过分离纯化蛋白激酶的上游激酶和磷酸酶，寻找新的生化调控途径，研究磷酸化及其调控的酶如何参与新的生理和病理过程。

 

代表性科研论文：

  1.  Li Y, Li CG, Liu YX, Yu JJ, Yang JQ, Cui YF, Wang TV, Li CY, Jiang LF, Song ML and Rao Y (2023). Sleep need, the key regulator of sleep homeostasis, is both indicated and controlled by phosphorylation of threonine 221 in salt inducible kinase 3. Genetics224:iyad136 (bioRxiv 2021.11.06.467421).
  2.  Dai X, Yang W, Zhang X, Zhou E, Liu Y, Wang T, Zhang WX, Zhang XX and Rao Y (2023). ppGpp is present in, and functions to regulate sleep of, Drosophila. hLife (bioRxiv2022.11.17.516975).
  3. Jia X, Zhu J, Bian X, Liu S, Yu S, Liang W, Jiang L, Mao R and Rao Y (2023) Importance of glutamine in synaptic vesicles revealed by functional studies of SLC6A17 and its mutations pathogenic for intellectual disability. eLife 12:RP86972 (bioRxiv2022.10.25.513688).
  4.  Liu Y, Shan L, Liu T, Li J, Chen Y, Sun C, Yang C, Bian X, Niu Y, Zhang C, Xi J and Rao Y (2023). Molecular and cellular mechanisms of the first social relationship: A conserved role of 5-HT from mice to monkeys, upstream of oxytocin. Neuron 111:1468-1685.
  5. Liu ZY, Jiang LF, Li CY, Li CG, Yang JQ, Yu JJ, Mao RB and Rao Y (2022). LKB1 is physiologically required for sleep from Drosophila melanogaster to the Mus musculus. Genetics 221:iyac082.
  6. Liu YX, Wang TV, Cui YF, Li CY, Jiang LF and Rao Y (2022). STE20 phosphorylation of AMPK related kinases revealed by biochemical purifications combined with genetics. J Biol Chem 298:101928.
  7. Liu YX, Wang TV, Cui YF, Gao SX and Rao Y (2022). Biochemical purification uncovers mammalian sterile 3 (MST3) as a new protein kinase for multifunctional protein kinases AMPK and SIK3.J Biol Chem 298:101929.
  8. Dai XHM, Zhou EX, Yang W, Deng BW, Li Q, Liu XX, Zhang WX and Rao Y (2021). Molecular resolution of a behavioral paradox: sleep and arousal are regulated by distinct acetylcholine receptors in different neuronal types in Drosophila. Sleep 10:1093.
  9. Zhu ZJ, Chen BQ, Na R, Fang W, Zhang WX, Zhou Q, Zhou SB, Lei H, Huang AL, Chen TM, Gao TM, Chen L, Ni DS, Gu YP, Liu JN, Chen JY, Rao Y and Fang F (2020). Heritability of human visual contour integration—an integrated genomic study. Eur J Hum Genet 27:1867-1875.
  10. Rao Y (2019). The First Hormone: Adrenaline. Trends Endocrinol Metab 30:331-334.
  11. Dai XHM, Zhou EX, Yang W, WX Zhang and Rao Y (2019). D-serine made by serine racemase in Drosophila intestine plays a physiological role in sleep. Nature Commun 10:1986.
  12. Deng BW, Li Q, Liu XX, Cao Y, Li BF, Qian YJ, Xu R, Mao RB, Zhou EX, Zhang WX, Huang J and Rao Y (2019). Chemoconnectomics: Mapping Chemical Transmission in Drosophila. Neuron 101: 876–893.
  13. 13.   Chen BQ, Zhu ZJ, Na R, Fang W, Zhou SB, Zhou Q, Lei H, Huang AL, Chen TM, Ni DS, Gu YP, Liu JN, Fang F and Rao Y (2018). Genomic Analyses of Visual Cognition: Perceptual Rivalry and Top-Down Control.  J Neurosci 38:9668 –9678.
  14. Zhu ZJ, Chen BQ, Yan HM, Fang W, Zhou Q, Zhou SB, Lei H, Huang AL, Chen TM, Gao TM, Chen L, Chen JY, Ni DS, Gu YP, Liu JN, Zhang WX and Rao Y (2018). Multi-level genomic analyses suggest new genetic variants involved in human memory. Eur J Hum Genet 26:1668-1678.
  15. Zhang X, Yan HM, Huang ZL and Rao Y (2018). Thermoregulation-Independent Regulation of Sleep by Serotonin Revealed in Mice Defective in Serotonin Synthesis. Mol Pharmacol 93:657-664.
  16. Chen BQ, Zhu, ZJ, Wang YY, Ding XH, Guo XB, He MG, Fang W, Zhou SB, Zhou Q, Huang AL, Chen TM, Ni DS, Gu YP, Liu JN, Lei H and Rao Y (2018). Nature vs. nurture in human sociality: multi-level genomic analyses of social conformity. J Hum Genet 63:605-619.
  17. Qian YJ, Cao Y, Deng BW, Yang G, Li J, Xu R, Zhang D, Huang J and Rao Y (2017). Sleep homeostasis regulated by 5HT2b receptor in a small subset of neurons in the dorsal fan-shaped body of Drosophila. eLife 6:e26519.
  18.  Zhu ZJ, Wang YY, Cao ZJ, Chen BQ, Cai HQ, Wu YH and Rao Y (2016). Cue-independent memory impairment by reactivation-coupled interference in human declarative memory. Cognitio,155:125–134.
  19. Zhang SS, Liu Y and Rao Y (2013). Serotonin signaling in the brain of adult female mice is required for sexual preference. Proc Natl Acad Sci USA 110: 9968-9973.
  20. Zhou C, Huang H, Kim SM, Lin H, Meng X, Chiang A-S, Wang JW, Jiao R and Rao Y (2012). Molecular genetic analysis of sexual rejection: roles of octopamine and its receptor OAMB in Drosophila courtship conditioning. J Neurosci 32:14281-14287.
  21.  Liu WW, Liang XH, Li YN, Gong JX, Yang Z, Zhang YH, Zhang JX and Rao Y (2011). Social regulation of aggression mediated by pheromonal activation of Or65a olfactory receptor neurons in Drosophila. Nat Neurosci 7:896-902.
  22.  Liu Y, Jiang Y, Si Y, Kim J-Y, Chen Z-F and Rao Y (2011). Molecular regulation of sexual preference revealed by genetic studies of 5-HT in the brain of male mice. Nature 472:95-99.
  23. Zhou C, Rao Y and Rao Y (2008). A subset of octopaminergic neurons are important for Drosophila aggression. Nat Neurosci 11:1059-1067.
  24.  Li X, Gao X, Liu G, Xiong W, Wu J and Rao Y (2008). Netrin signal transduction and the guanine nucleotide exchange factor DOCK180 in attractive signaling. Nat Neurosci 11:28-35.
  25.  Guo W, Jiang H, Gray V, Dedhar S and Rao Y (2007). Role of the integrin-linked kinase (ILK) in determining neuronal polarity. Dev Biol 306:457-468.
  26. Jiang H, Guo W, Liang XH and Y Rao (2005). Both the establishment and the maintenance of neuronal polarity require active mechanisms: critical roles of GSK-3b and its upstream regulators. Cell 120:123-135.
  27. Liu G, Beggs H, Jürgensen C, Park HT, Tang H, Gorski J, Jones KR, Reichardt LF, Wu JY and Rao Y (2004). Netrin requires the focal adhesion kinase and the Src family kinases to induce axon outgrowth and to attract axons. Nat Neurosci 7:1222-1232.
  28. Ward ME, Wu JY and Rao Y (2004). Visualization of spatially and temporally regulated N-WASP activity during cytoskeletal reorganization in living cells. Proc Natl Acad Sci 101:970-974.
  29. Zhu Y, Yu T, Zhang X-C, Nagasawa T, Wu JY and Rao Y (2002). Role of the chemokine SDF-1 as the meningeal attractant for embryonic cerebellar neurons. Nat Neurosci. 5:719-720.
  30. 30.   Wong K, Ren X-R, Huang Y-Z, Xie Y, Liu G, Saito H, Tang H, Wen L, Brady-Kalnay SM, Mei L, Wu JY, Xiong W-C and Rao Y (2001). Signal transduction in neuronal migration: roles of GTPase activating proteins and the small GTPase Cdc42 in the Slit-Robo pathway. Cell 107:209-221.
  31. Wu JY, Feng L, Park H-T, Havlioglu N, Wen L, Tang H, Bacon KB, Jiang Z, Zhang X-C and Rao Y (2001). The neuronal repellent Slit inhibits leukocyte chemotaxis induced by chemotactic factors. Nature 410:948-952.
  32. Wu W, Wong K, Chen JH, Jiang ZH, Dupuis S, Wu JY and Rao Y (1999). Directional guidance of neuronal migration in the olfactory system by the secreted protein Slit. Nature 400:331-336.
  33. Li HS, Chen JH, Wu W, Fagaly T, Yuan WL, Zhou L, Dupuis S, Jiang Z, Nash W, Gick C, Ornitz D, Wu JY and Rao Y (1999). Vertebrate Slit, a secreted ligand for the transmembrane protein Roundabout, is a repellent for olfactory bulb axons. Cell 96:807-818. 
  34. Li HS, Tierney C, Wen L, Wu JY and Rao Y (1997). A single morphogenetic field gives rise to two retina primordia under the influence of the prechordal mesoderm. Development 124:603-615. 
  35. Rao Y, Jan LY, and Jan YN (1990). Similarity of the product of the Drosophila neurogenic gene big brain to transmembrane channel proteins. Nature 345:163-167. 

 

实验室网站：

www.raolab.org