Research Interests:
My research interest falls into the area of metabolism and developmental biology. In particular, I am interested in how the adipose and intestinal tissues respond to dietary and temperature interventions at the transcriptomic and lipidomic levels, and the cellular growth pattern and molecular mechanisms that drive and regulate formation of the intervertebral disc during embryonic development. The methods that will be employed to address these questions include, but not limited to single-cell/nucleus sequencing, spatial sequencing, liquid chromatography/mass spectrometry and genetic lineage tracing methods etc. Model organisms to be used include mouse, zebrafish, human and monkey samples etc.
Selected Publications:
1. Xie M, Gol'din P, Herdina AN, Estefa J, Medvedeva EV, Li L, Newton PT, Kotova S, Shavkuta B, Saxena A, Shumate LT, Metscher BD, Großschmidt K, Nishimori S, Akovantseva A, Usanova AP, Kurenkova AD, Kumar A, Arregui IL, Tafforeau P, Fried K, Carlström M, Simon A, Gasser C, Kronenberg HM, Bastepe M, Cooper KL, Timashev P, Sanchez S, Adameyko I, Eriksson A, Chagin AS. Secondary ossification center induces and protects growth plate structure. Elife. 2020 Oct 16;9:e55212. doi: 10.7554/eLife.55212.
2. Xie M, Chagin AS. The epiphyseal secondary ossification center: Evolution, development and function. Bone. 2020 Oct 19:115701. doi: 10.1016/j.bone.2020.115701.
3. Zhang Y, Annusver K, Sunadome K, Kameneva K, Edwards S, Kasper M, Chagin AS, Adameyko I*, Xie M*. Epiphyseal cartilage formation involves differential dynamics of various cellular populations during embryogenesis. Frontiers in Cell and Developmental Biology. 2020 Mar 5;8:122. doi: 10.3389/fcell.2020.00122. eCollection 2020. *Corresponding authors
4. Zhang Y, Kameneva P, Annusver K, Kasper M, Chagin AS, Adameyko I*, Xie M*. Cruciate ligament, patellar tendon and patella formation involves differential cellular sources and dynamics as joint cavitation proceeds. Developmental Dynamics. 2020 Jun;249(6):711-722. doi: 10.1002/dvdy.158. * Corresponding authors
5. Xie M, Kamenev D, Kaucka M, Kastriti ME, Zhou B, Artemov AV, Storer M, Fried K, Adameyko I, Dyachuk V, Chagin AS. Schwann cell precursors contribute to skeletal formation during embryonic development in mice and zebrafish. Proc Natl Acad Sci U S A. 2019 Jul 23;116(30):15068-15073. doi: 10.1073/pnas.1900038116.
6. Xie M, Li JP. Heparan sulfate proteoglycan - A common receptor for diverse cytokines. Cell Signal. 2019 Feb;54:115-121. doi: 10.1016/j.cellsig.2018.11.022.
7. Xie M and Roy R. AMP-Activated Kinase Regulates Lipid Droplet Localization and Stability of Adipose Triglyceride Lipase in C. elegans Dauer Larvae. PLoS One. 2015 Jun 22;10(6):e0130480. doi: 10.1371/journal.pone.0130480.
8. Xie M and Roy R. The Causative Gene in Chanarian-Dorfman Syndrome Regulates Lipid Droplet Homeostasis in C. elegans. PLoS Genetics. 2015 Jun 17;11(6):e1005284. doi: 10.1371/journal.pgen.1005284.
9. Xie M and Roy R. Increased Levels of Hydrogen Peroxide Induce a HIF-1-dependent Remodelling of Lipid Metabolism in C. elegans. Cell Metabolism. 2012 Sep 5;16(3):322-35.
10. Xie M, Alonso H and Roujeinikova A. An Improved Procedure for the Purification of Catalytically Active Alkane Hydroxylase from Pseudomonas putida GPo1. Appl Biochem Biotechnol. 2011 Oct;165(3-4):823-3.