Principal Investigator

Zhuan Zhou
Exocytosis and Endocytosis, Ion Channel, Dopamine, Calcium Imaging in Vivo, Drug Addiction




Research Interests:

Secretion is a principle function of a cell. Neurotransmitter and hormone secretion is triggered by increase in intracellular Ca concentration. We investigate mechanisms of neurotransmitter release from synapse and/or soma of a cell by patch-clamp, membrane capacitance and carbon fiber electrodes and fluorescent optic measurements at 3 physiological levels: single cells, brain slices, and in vivo.



Selected Publications:

1. Wang CH, Kang XJ, Zhou L, Chai ZY, Wu QH, Huang R, Xu HD, Hu MQ, Sun XX, Sun SH, Li J, Jiao RY, Zuo PL, Zheng LH, Yue ZY, and Zhou Z*. Synaptotagmin-11 is a critical mediator of parkin linked neurotoxicity and Parkinson’s Disease-like pathology. Nature Communications, 2018,9(1):81.

2. Chai ZY, Wang CH, Huang R, Wang Y, Zhang XY, Wu QH, Wang YS, Wu X, Zheng LH, Zhang C, Guo W, Xiong W, Ding JP, Zhu FP, and Zhou Z*. CaV2.2 Gates Calcium-Independent but Voltage-Dependent Secretion in Mammalian Sensory Neurons. Neuron, 2017, 96(6):1317-1326.

3. Wang Y, Wu Q, Hu M, Liu B, Chai Z, Huang R, Wang Y, Xu H, Zhou L, Zheng L, Wang C, Zhou Z*. Ligand- and voltage-gated Ca2+ channels differentially regulate the mode of vesicular neuropeptide release in mammalian sensory neurons. Science Signaling, 2017, 10(484):1-7.

4. Shang SJ, Zhu FP, Liu B, Chai ZY, Wu QH, Hu MQ, Wang Y, Huang R, Zhang ZY, Wu X, Sun L, Wang YS, Wang L, Xu HD, Teng SS, Liu B, Zheng LH, Zhang C, Zhang FK, Feng XH, Zhu DS, Wang CH, Liu T, Zhu MX, and Zhou Z*. Intracellular TRPA1 mediates Ca2+ release from lysosomes in dorsal root ganglion neurons. J Cell Biol, 2016, 215(3):369-381.

5. Wang CH, Wang YS, Hu MQ, Chai ZY, Wu QH, Huang R, Han WP, Zhang CX and Zhou Z. A synaptotagmin negatively regulates dynamin dependent clathrin-mediated and bulk endocytosis. EMBO Reports, 2016, 17(1), 47-63.

6. Shang CF, Li XQ, Yin C, Liu B, Wang YF, Zhou Z*, Du JL. Amperometric Monitoring of Sensory-Evoked Dopamine Release in Awake Larval Zebrafish. J Neurosci, 2015, 35(46):15291-4.

7.He LL, Zhang QF, Wang LC, Dai JX, Wang CH, Zheng LH, Zhou Z*. Muscarinic inhibition of nicotinic transmission in rat sympathetic neurons and adrenal chromaffin cells. Philos Trans R Soc Lond B Biol Sci, 2015, 370(1672).

8. Dou H, Wang C, Wu X, Yao L, Zhang X, Teng S, Xu H, Liu B, Wu Q, Zhang Q, Hu M, Wang Y, Wang L, Wu Y, Shang S, Kang X, Zheng L, Zhang J, Raoux M, Lang J, Li Q, Su J, Yu X, Chen L and Zhou Z*. Calcium influx activates local adenylyl cyclase 8 for sustained insulin secretion in rat pancreatic β cells. Diabetologia, 2015, 58(2):324-33.

9. Kang, X., H. Xu, S. Teng, X. Zhang, Z. Deng, L. Zhou, P. Zuo, B. Liu, Q. Wu, L. Wang, M. Hu, H. Dou, W. Liu, F. Zhu, Q. Li, S. Guo, J. Gu, Q. Lei, J. Lu, Y. Mu, M. Jin, S. Wang, W. Jiang, K. Liu, C. Wang, W. Li, K. Zhang, and Z. Zhou*. Dopamine release from transplanted neural stem cells in Parkinsonian rat striatum in vivo. Proc Natl Acad Sci USA, 2014, 111:15804-9.

10. Wang L, Xu HD, Zhou L, Jiao RY, Liu W, Zhu FP, Kang XJ, Zhang XY, Liu B, Teng SS, Wu QH, Li ML, Dou HQ, Wang CH, Zuo PL, Wang SR and Zhou Z*. Temporal components of synapse-synapse transmission in dorsal striatum brain slices. Journal of Physiology, 2014, 592:3559–3576.

11. Wang L, Shang SJ, Kang XJ , Teng SS, Zhu FP, Liu B, Wu QH, Li ML, Liu W, Xu HD, Zhou L, Jiao RY, Dou HQ, Zuo PL, Zhang XY, Zheng LH, Wang SR, Wang CH and Zhou Z*. A mechanism of modulating dopamine release in the striatum by smoking-level nicotine. Nature Communications, 2014, 5:3925.

12. Chen XW, Feng YQ, Hao CJ, Guo XL, He X, Zhou ZY, Guo N, Huang HP, Xiong W, Zheng H, Zuo PL, Zhang CX, Li W, Zhou Z*. A Schizophrenia-Susceptible Gene Affects Quanta Size of Transmitter Release. J Cell Biol, 2008, 181: 791-801.

13. Mu Y, Guo N, Fan SY, Wang SR, Huang W, Zheng LH, Li LH, Hu ZA, Zhou Z*. Hypocretin-1 potentiates NMDA receptor-mediated somatodendritic secretion from locus coeruleus neurons. J. Neurosci. , 2008, 28:3202-08.

14. Huang HP, Wang SR, Yao W, Zhang C, Zhou Y, Zhang B, Xiong W, Chen XW, Zheng LH, Wang LY, Marc Landry, Hokfelt T, Xu ZQD and Zhou Z*. Long latency of evoked quantal transmitter release from somata of locus coeruleus neurons in rat pontine slices. Proc Natl Acad Sci USA.,2007, 104(4):1401-1406.

15. Chen XK, Wang LC, Zhou Y, Zheng LH and Zhou Z*. “Kiss-and-Run” Glutamate Secretion in Cultured and Freshly Isolated Rat Hippocampal Astrocytes. J. Neurosci., 2005, 25(40):9236 –9243.

16. Chen XK, Wang LC, Zhou Y, Cai Q, Prakriya M, Duan KL, Sheng ZH, Lingle C, Zhou Z*. Activation of GPCRs modulates quantal size in chromaffin cells through Gbc and PKC. Nature Neuroscience, 2005, 8(9):1160 - 1168.

17.Zhang C, Xiong W, Zheng H, Wang L, Lu B and Zhou Z*. Calcium- and dynamin-independent endocytosis in dorsal root ganglion neurons. Neuron, 2004, 42: 225–236.

18.Yu X, Duan KL, Shang CF, Yu HG and Zhou Z*. Calcium influx through hyperpolarization-activated cation channels ( Ih channels) contributes to activity-evoked neuronal secretion. Proc Natl Acad Sci USA., 2004, 101:1051-1056.

19.Duan KL, Yu X, Zhang C, and Zhou Z*. Control of Secretion by Temporal Patterns of Action Potentials in Adrenal Chromaffin Cells. J. Neurosci., 2003, 23(35):11235-43.

20.Zhang C and Zhou Z*.Ca(2+)-independent but voltage-dependent secretion in mammalian dorsal root ganglion neurons. Nat Neurosci..2002, 5(5): p. 425-30.



Lab Website: