Principal Investigator

Zhuo Huang
Ion channels、epilepsy, depression, dendritic ion channels, axonal ion channels, drug screening, patch-clamp recordings

phone:
address: 1501, Medical Science and Technology Building, 38# College Road, Haidian District, Beijing
email: huangz@hsc.pku.edu.cn

 

Research Interests:

Ion channels have to be at the right place in the right number to endow individual neurons with their specific character. My lab in Peking University utilized dendritic and axonal patch-clamp recordings combined with calcium-sensitive dye two-photon imaging, molecular biology, genetics and pharmacological techniques to uncover the expression and function of ion channels in neuronal compartments such as dendrites, axon…….

 

Selected Publications:

 

  1. Yuan T, Wang YF, Jin YC, Xu S, Zhang H, Chen Q, Li N, Ma XY, Song HF, Peng C, Yang H, Geng Z, Dong J, Duan GF, Sun Q, Yang Y, Yang F, Huang Z* (2023) Coupling of Slack and NaV1.6 sensitizes Slack to quinidine blockade and guides anti-seizure strategy development. eLife. 12:RP87559
  2. Fan MH, Liu YQ, Shang YF, Xue YX, Liang J, Huang Z* (2022) JADE2 is essential for hippocampal synaptic plasticity and cognitive, Biological Psychiatry. 2022 Nov 15;92(10):800-814.
  3. Liu YQ, Li MH, Fan MH, Song Y, Yu HJ, Zhi XJ, Xiao K, Lai SR, Zhang JL, Jin XQ, Shang YF, Liang J*, and Huang Z* (2019) CDYL-mediated histone crotonylation regulates stress-induced depressive behaviors, Biological Psychiatry, 85(8):635-649
  4. Liu YQ, Lai SR, Ma WN, Ke W, Zhang C, Liu SM, Zhang Y, Pei F, Li SY, Yi M, Shu YS, Shang YF, Liang J*, and Huang Z* (2017) CDYL suppresses epileptogenesis through repression of axonal Nav1.6 sodium channel expression. Nature Communications, 8(1):355.
  5. Gao, Y., Xu, S., Cui, X., Xu H., Qiu Y., Wei Y., Dong Y., Zhu B., Peng C., Liu S., Zhang X., Sun J., Huang Z*, Zhao Y. (2023) Molecular insights into the gating mechanisms of voltage-gated calcium channel CaV2.3.  Nature Communications. 14, 516 (2023). https://doi.org/10.1038/s41467-023-36260-2
  6. Qu Y, Lu Y, Zhang D, Liu X, Fan P, Chen J, Zhang H, Yang K, Tian T, Zhou Y, Zhang Q, Zhang Y, Wang L, Huang Z*, Liu Y*, Hu A*, Zhou X*. (2023) Identification of a novel frameshift mutation in the SCNN1B causing Liddle syndrome. Science Bulletin (Beijing). 2023 Feb 7:S2095-9273(23)00091-9. doi: 10.1016/j.scib.2023.02.006. Epub ahead of print. PMID: 36774301.
  7. Li Y., Yuan T., Huang B., Zhou F., Peng C., Li X., Qiu Y., Yang B., Zhao Y., Huang Z.*, and Jiang D.* (2023) Structure of human NaV1.6 channel reveals Na+ selectivity and pore blockade by 4,9-anhydro-tetrodotoxin. Nature Communications.
  8. Zhang J, Shi Y, Fan J, Chen H, Xia Z, Huang B, Jiang J, Gong J, Huang Z*, Jiang D*. (2022) N-type fast inactivation of a eukaryotic voltage-gated sodium channel. Nature Communications. 2022 May 17; 13(1):2713.
  9. Zhang ZL, Chen HW, Geng Z, Yu ZY, Li H, Dong YL, Zhang HW, Huang Z*, Jiang JQ*, Zhao Y* (2022) Structural basis of ligand binding modes of human EAAT2, Nature Communications. Jun 9;13(1):3329.
  10. Dong Y, Gao Y, Xu S, Wang Y, Yu Z, Li Y, Li B, Yuan T, Yang B, Zhang XC, Jiang D*, Huang Z*, Zhao Y*. (2021) Closed-state inactivation and pore-blocker modulation mechanisms of human CaV2.2. Cell Rep. Nov 2;37(5):109931.
  11. Li Z, Cai G, Fang F, Li W, Fan M, Lian J, Qiu Y, Xu X, Lv X, Li Y, Zheng R, Wang Y, Li Z, Zhang G, Liu Z, Huang Z*, Zhang L*. Discovery of Novel and Potent Methyl-d-aspartate Receptor Positive Allosteric Modulators with Antidepressant-like Activity in Rodent Models (2021). J Med Chem. 13;64(9):5551-5576.
  12. Zhang J, Zhang C, Chen X, Wang B, Ma W, Yang Y, Zheng R*, Huang Z* (2021) PKA-RIIβ autophosphorylation modulates PKA activity and seizure phenotypes in mice. Communication Biology, 4(1):263.
  13. Ma Y, Xie H, Du X, Wang L, Jin X, Zhang Q, Han Y, Sun S, Wang L, Li X, Zhang C, Wang M, Li C, Xu J, Huang Z*, Wang X*, Chai Z*, Deng H*. (2021) In vivo chemical reprogramming of astrocytes into neurons. Cell Discovery 7(1):12.
  14. Kong W, Tu X, Huang W, Yang Y, Xie Z* & Huang Z * (2020) Prediction and Optimization of NaV1.7 Sodium Channel Inhibitors Based on Machine Learning and Simulated Annealing. Journal of chemical information and modeling, 60(6), 2739–2753.
  15. Chi XM, Jin XQ, Chen Y, Lu XL, Tu XY, Li XR, Zhang YY, Lei JL, Huang J, Huang Z*, Zhou Q* and Pan XJ* (2020) Structural insights into the gating mechanism of human SLC26A9 mediated by its C-terminal sequence, Cell Discovery, 6:55
  16. Yang L, Liu Y, Fan M, Zhu G, Jin H, Liang J, Liu Z, Huang Z*, Zhang L* (2019) Identification and characterization of benzo[d]oxazol-2(3H)-one derivatives as the first potent and selective small-molecule inhibitors of chromodomain protein CDYL, Eur J Med Chem., 15;182:111656
  17. Huang Z, Lujan R, Kadurin I, Uebele VN, Renger JJ, Dolphin AC, Shah MM. (2011) Presynaptic HCN1 channels regulate Ca(V)3.2 activity and neurotransmission at select cortical synapses, Nature Neuroscience, 14(4):478-86 
  18. Huang Z, Lujan R, Martinez-Hernandez J, Lewis AS, Chetkovich DM, Shah MM. (2012) TRIP8b-Independent Trafficking and Plasticity of Adult Cortical Presynaptic HCN1 Channels. Journal of Neuroscience, 32(42):14835-48. 
  19. Huang Z, Walker MC, Shah MM. (2009) Loss of dendritic HCN1 subunits enhances cortical excitability and epileptogenesis, Journal of Neuroscience, 29(35):10979-88. 

 

Lab Website:

http://dmcp.sps.bjmu.edu.cn/szdw_20180115155901250603/tpyjy_20180115155901250603/192175.htm