题目：Fine live imaging with light for neuroscience research

　　主讲人：Jianan Y. QU（瞿佳男）, Ph.D.

　　Biophotonics Research Laboratory,

　　Biomedical Engineering Program,

　　the Department of Electronics and Computer Engineering,

　　Hong Kong University of Science and Technology

　　时间：2022年6月24日（星期五），14:00-16:00

　　腾讯会议：770-121-324

　　会议密码：666666

　　主持人：李毓龙教授

　　学生主持: 易馨阳

　　Abstract：

　　The direct and non-invasive visualization of neurons, glia, and microvasculature in the central nervous system (CNS) in vivo is critical for enhancing our understanding of how the CNS functions and diseases develop/progress in CNS.  Over recent decades, great effort has been focused on developing novel techniques for in vivo imaging of the intact CNS. However, none of the prevalent technologies, including ultrasound imaging (sonography), positron emission tomography (PET), and magnetic resonance imaging (MRI), provides sufficient spatial resolution to visualize biological structures at the subcellular level. Optical microscopy has greatly facilitated biomedical research in recent decades, with its ability to provide structural and functional information in living specimens at high spatiotemporal resolution. Particularly, the multi-photon microscopy (MPM) has been developed by using longer wavelengths and higher-order nonlinear excitation. It has demonstrated great potential to extend the imaging depth. However, optical aberration and scattering occur as light travels through and interacts with inhomogeneous biological tissues, such as the mammalian brain and spinal cord, fundamentally limiting the performance of optical microscopy in both resolution and depth. In recent years, our research efforts have been focused on the development of a variety of adaptive optics (AO) technologies that allow fast measurement and correction of both low and high order aberrations in tissue and mouse eye optical system. We demonstrated that the AO-MPM technology enables accurate measurement of aberrated laser focus in tissue and fast correction of the aberration to achieve near non-invasive live imaging of retina, spinal cord and brain at unprecedented depth and resolution. Our results and findings show that the innovative AO-MPM technology holds great potential to advance in vivo imaging techniques and facilitate neuroscience study in CNS.