研究兴趣：

细胞的分泌是细胞最基本的一个功能。细胞内钙离子的升高将引起神经递质和激素的分泌。我们研究分泌的分子和细胞机制，以及多巴胺分泌生理学及其与神经退行性疾病的关系。使用膜片钳、膜电容、微碳纤电极以及共聚焦、多光子荧光显微成像在活体（in vivo）、组织（brain slices）、单细胞水平测量检测量子化神经递质、激素(特别是儿茶酚胺类)分泌信号。

代表性科研论文：

1. Wang L, Gao M, Wang Q, Sun L, Younus M, Ma S, Liu C, Shi L, Lu Y, Zhou B, Sun S, Chen G, Li J, Zhang Q, Zhu F, Wang C, Zhou Z. Cocaine induces locomotor sensitization through a dopamine-dependent VTA-mPFC-FrA cortico-cortical pathway in male mice.  Nature Communications, 2023,14(1):1568. doi: 10.1038/s41467-023-37045-3..  

2. Wang Y, Huang R, Chai Z, Wang C, Du X, Hang Y, Xu Y, Li J, Jiang X, Wu X, Qiao Z, Li Y, Liu B, Zhang X, Cao P, Zhu F, Zhou Z. Ca2+ -independent transmission at the central synapse formed between dorsal root ganglion and dorsal horn neurons. EMBO Reports, 2022,23(11): e54507. doi: 10.15252/embr.202154507 .  

3. Lin Z, Li Y, Hang Y, Wang C, Liu B, Li J, Yin L, Jiang X, Du X, Qiao Z, Zhu F, Zhang Z, Zhang Q, Zhou Z. Tuning the Size of Large Dense-Core Vesicles and Quantal Neurotransmitter Release via Secretogranin II Liquid-Liquid Phase Separation. Advanced Science, 2022,9(27): e2202263. doi: 10.1002/advs.202202263.  

4. Zhu F, Liu L, Li J, Liu B, Wang Q, Jiao R, Xu Y, Wang L, Sun S, Sun X, Younus M, Wang C, Hokfelt T, Zhang B, Gu H, Xu ZD, Zhou Z  Cocaine increases quantal norepinephrine secretion through NET-dependent PKC activation in locus coeruleus neurons. Cell Reports, 2022,40(7):111199. doi: 10.1016/j.celrep.2022.111199.  

5. Quanfeng Zhang , Bin Liu , Qihui Wu, Bing Liu , Yinglin Li , Suhua Sun, Yuan Wang, Xi Wu, Zuying Chai, Xiaohan Jiang, Xiaoyao Liu, Meiqin Hu, Yeshi Wang, Yunting Yang, Li Wang, Xinjiang Kang, Yingfei Xiong, Yang Zhou, Xiaoke Chen, Lianghong Zheng, Bo Zhang, Changhe Wang, Feipeng Zhu, Zhuan Zhou*, “Differential co-release of two neurotransmitters from a vesicle fusion pore in mammalian adrenal chromaffin cells.” Neuron,102(1):173-183 (2019).

6. Quanfeng Zhang, Bing Liu, Yinglin Li, Lili Yin, Muhammad Younus, Xiaohan Jiang, Zhaohan Lin, Xiaoxuan Sun, Rong Huang, Bin Liu, Qihui Wu, Feipeng Zhu, Zhuan Zhou*,“Regulating quantal size of neurotransmitter release through a GPCR voltage sensor.” Proc Natl Acad Sci U S A, 2020,117(43):26985-26995.

7. Mingli Li, Huadong Xu, Guoqing Chen, Suhua Sun, Qinglong Wang, Bing Liu, Xi Wu, Li Zhou, Zuying Chai, Xiaoxuan Sun, Yang Lu, Muhammad Younus, Lianghong Zheng, Feipeng Zhu, Hongbo Jia, Xiaowei Chen, Changhe Wang, Zhuan Zhou*,“Impaired D2 receptor-dependent dopaminergic transmission in prefrontal cortex of awake mouse model of Parkinson's disease.” Brain, 2019,142(10):3099-3115.

8. Rong Huang, Yuan Wang, Jie Li, Xiaohan Jiang, Yinglin Li, Bing Liu, Xi Wu, Xingyu Du, Yuqi Hang, Mu Jin, Feipeng Zhu, Changhe Wang*, Zuying Chai*, Zhuan Zhou*,“Ca 2+-independent but voltage-dependent quantal catecholamine secretion (CiVDS) in the mammalian sympathetic nervous system.”Proc Natl Acad Sci U S A, 2019,116(40):20201-20209.

10. Changhe Wang, Xinjiang Kang, Li Zhou, Zuying Chai, Qihui Wu, Rong Huang, Huadong Xu, Meiqin Hu, Xiaoxuan Sun, Suhua Sun, Jie Li, Ruiying Jiao, Panli Zuo, Lianghong Zheng, Zhenyu Yue, Zhuan Zhou*,“Synaptotagmin-11 is a critical mediator of parkin-linked neurotoxicity and Parkinson's disease-like pathology.” Nature Communications, 2018,9(1):81.

11. Zuying Chai, Changhe Wang, Rong Huang, Yuan Wang, Xiaoyu Zhang, Qihui Wu, Yeshi Wang, Xi Wu, Lianghong Zheng, Chen Zhang, Wei Guo, Wei Xiong, Jiuping Ding, Feipeng Zhu, Zhuan Zhou*,“CaV2.2 Gates Calcium-Independent but Voltage-Dependent Secretion in Mammalian Sensory Neurons.” Neuron, 2017,96(6):1317-1326.

12. Li Wang, Shujiang Shang, Xinjiang Kang, Sasa Teng, Feipeng Zhu, Bin Liu, Qihui Wu, Mingli Li, Wei Liu, Huadong Xu, Li Zhou, Ruiying Jiao, Haiqiang Dou 2, Panli Zuo, Xiaoyu Zhang, Lianghong Zheng, Shirong Wang, Changhe Wang, Zhuan Zhou*,“Modulation of dopamine release in the striatum by physiologically relevant levels of nicotine.” Nature Communications, 2014,5:3925.

13. Xiao-Ke Chen, Lie-Cheng Wang, Yang Zhou, Qian Cai, Murali Prakriya, Kai-Lai Duan, Zu-Hang Sheng, Christopher Lingle, Zhuan Zhou*,“Activation of GPCRs modulates quantal size in chromaffin cells through G(betagamma) and PKC.” Nature Neuroscience, 2005,8(9):1160-1168.

14. Zhang C & Zhou Z*, “Ca(2+)-independent but voltage-dependent secretion in mammalian dorsal root ganglion neurons.”  Nature Neuroscience, 5(5):425-30(2002).

15. Chen Zhan g, Wei Xiong, Hui Zheng, Liecheng Wang, Bai Lu, Zhuan Zhou*,“Calcium- and dynamin-independent endocytosis in dorsal root ganglion neurons.” Neuron, 2004,42(2): 225-36.

16. Hao Qian, Xinjiang Kang, Jing Hu, Dongyang Zhang, Zhengyu Liang, Fan Meng, Xuan Zhang, Yuanchao Xue, Roy Maimon, Steven F. Dowdy, Neal K. Devaraj, Zhuan Zhou, William C. Mobley, Don W. Cleveland & Xiang-Dong Fu, Rversing a model of Parkinson’s disease with in situ converted nigral neurons. Nature, 2020,582(7813):550-556. doi: 10.1038/s41586-020-2388-4. (my lab contribute the in vivo recordings of dopamine release in PD vs. WT mice. Fig. 5f-k）

实验室网站：

https://future.pku.edu.cn/jsdw/jy/fzyxs1/a19025ef2c4d46428c2d6c2ac0a61250.htm