Principal Investigator

李毓龙
荧光探针、神经成像、突触传递、信号转导、神经疾病

联系电话:62766905
通信地址: 北京市海淀区颐和园路5号金光生命科学大楼142信箱,100871
电子邮件:yulongli@pku.edu.cn

李毓龙,研究员

 

研究兴趣:

人的大脑有超过几十亿个神经元。这些神经元分为上千个种类,并通过上千亿个突触相互联系。我们发展高效的可遗传编码的荧光分子探针,研究新的成像的方法,并结合两者研究神经元细胞水平上的信号转导,分析神经环路中细胞可兴奋性及突触传递调控的机制。我们还用发展的可视化方法探索在疾病模型中的神经系统环路上的可能的病变及机能障碍。高灵敏的光学成像技术可以提供重要的时空分辨率,多路观测可以同时对多个神经元成像,这些新的方法对于揭开大脑神经调质的特异性的细胞基础提供了有效的方法。我们希望我们新的技术发展能让更多的神经生物学家能用简单有效的方法分析神经局部环路中复杂的突触的活动,解析大脑精巧的结构与功能的关系。

 

 

代表性科研论文:

1. #Wu, Z., #Feng, J., Jing, M., & *Li, Y. (2019). G protein-assisted optimization of GPCR-activation based (GRAB) sensors. Neural Imaging and Sensing 2019, vol. 10865, p. 108650N. International Society for Optics and Photonics.

2. #Zhou, M., #Chen, N., Tian, J., Zeng, J., ... Li, Y., *Guo, A., & *Li, Y. (2018). Suppression of GABAergic neurons through D2-like receptor secures efficient conditioning in Drosophila aversive olfactory learning. (2019). PNAS, 201812342.

3. Wu, L., Dong, A., Dong, L., Wang, S. Q., & *Li, Y. (2019). PARIS, an optogenetic method for functionally mapping gap junctions. eLife, 8, e43366.

4. Li, B., Wong, C., Gao, S. M., Zhang, R., Sun, R., Li, Y., & *Song, Y. (2018). The retromer complex safeguards against neural progenitor-derived tumorigenesis by regulating Notch receptor trafficking. eLife, 7, e38181.

5. Tanaka, M., Sun, F., Li, Y., & *Mooney, R. (2018). A mesocortical dopamine circuit enables the cultural transmission of vocal behaviour. Nature, 563(7729), 117-120.

6. #Sun, F., #Zeng, J., #Jing, M., Zhou, J., Feng, J., Owen, S., ... & *Li, Y. (2018). A genetically-encoded fluorescent sensor enables rapid and specific detection of dopamine in flies, fish, and mice. Cell, 174(2), 481-496.

7. #Jing, M., #Zhang, P., Wang, G., Feng, J., Mesik, L., Zeng, J., ... *Zhu, JJ & *Li, Y. (2018). A genetically-encoded fluorescent acetylcholine indicator for in vitro and in vivo studies. Nature Biotechnology, 36(8), 726-737.

8. #Chen, B., #Huang, X., Gou, D., Zeng, J., ... & Li, Y., *Chen, L., *Wang, A. (2018). Rapid volumetric imaging with Bessel-Beam three-photon microscopy. Biomedical optics express, 9(4), 1992-2000.

9. Shen, Y., Ge, W. P., Li, Y., Hirano, A., Lee, H. Y., Rohlmann, A., ... *Fu, Y. H. & *Ptacek, L. J. (2015). Protein mutated in paroxysmal dyskinesia interacts with the active zone protein RIM and suppresses synaptic vesicle exocytosis. Proceedings of the National Academy of Sciences, 112(10), 2935-2941.

10. Liang, L., Li, Y., Potter, C. J., Yizhar, O., Deisseroth, K., Tsien, R. W., & *Luo, L. (2013). GABAergic Projection Neurons Route Selective Olfactory Inputs to Specific Higher-Order Neurons. Neuron, 79(5), 917-931.

11. *Li, Y., & *Tsien, R. W. (2012). pHTomato, a red, genetically encoded indicator that enables multiplex interrogation of synaptic activity. Nature neuroscience, 15(7), 1047-1053.

12. Park, H., Li, Y., & *Tsien, R. W. (2012). Influence of synaptic vesicle position on release probability and exocytotic fusion mode. Science, 335(6074), 1362-1366.

13. *Yoo, A. S., Sun, A. X., Li, L., Shcheglovitov, A., Portmann, T., Li, Y., ... & *Crabtree, G. R. (2011). MicroRNA-mediated conversion of human fibroblasts to neurons. Nature, 476(7359), 228-231.

14. Zhang, Q., Li, Y., & *Tsien, R. W. (2009). The dynamic control of kiss-and-run and vesicular reuse probed with single nanoparticles. Science, 323(5920), 1448-1453.

15. *Kuner, T., Li, Y., Gee, K. R., Bonewald, L. F., & Augustine, G. J. (2008). Photolysis of a caged peptide reveals rapid action of N-ethylmaleimide sensitive factor before neurotransmitter release. Proceedings of the National Academy of Sciences, 105(1), 347-352.

16. Li, Y., Augustine, G. J., & *Weninger, K. (2007). Kinetics of complexin binding to the SNARE complex: correcting single molecule FRET measurements for hidden events. Biophysical journal, 93(6), 2178-2187.

 

评论 & 亮点:

1. *Dong, A., Liu, S., & *Li, Y. (2018). Gap Junctions in the Nervous System: Probing Functional Connections Using New Imaging Approaches. Frontiers in Cellular Neuroscience, 12, 320.

2. Wang, H., Jing, M., & *Li, Y. (2018). Lighting up the brain: genetically encoded fluorescent sensors for imaging neurotransmitters and neuromodulators. Current Opinion in Neurobiology, 50, 171-178.

3. #Wang, A., #Feng, J., *Li, Y., & *Zou, P. (2018). Beyond Fluorescent Proteins: Hybrid and Bioluminescent Indicators for Imaging Neural Activities. ACS chemical neuroscience, 9(4), 639-650.

4. Qian, C., & *Li, Y. (2015). Spine maturation and pruning during development: Cadherin/Catenin complexes come to help. Science China. Life sciences,58(9), 929.

5. *Li, Y., & *Rao, Y. (2015). Pied Piper of Neuroscience. Cell, 163(2), 267-268.

 

 

实验室网站:

http://www.yulonglilab.org/